f:y [vsQSpeN'Y|SOb__Qs(WO\U^ N ~2 4 L0SpeTpe
f
[-NT[~_gT O\U^l:N>f:y!k[nvvsQOo`0
4 . 7 w8h~ހT~̃ukv5ulS
[Ǒ(u N!k'`v5uQog uirf[7hT>e(W5ugv -N07hTSOyugq5uQog
Nvh:ySOy0}6q5ug
NvmSO
NOf>fq_T[ FO;`SOHesO NM0
5uQogvxxzSO\OS0
5uQogvQXezwSOogP[ ccknxeTceQ5uQog^-N0ogvSvOOogP[0FO/f [hQw MQǏ'YSR vP[TogP[
N[\*Y%N0R}Y7hTv5uQog(WlЏ-NOWveT NMQ7hToQ0
V4 5uQogv~gV
bOOzQbKbSQ5uQog0R7hT5uQog-N
N gll0
S_5uQog>eeQlSi-Ne _{laOogP[vzwMONe -N ogP[c0Riv^0[hQc}Y5uQogvlSiMbScNhV^ۏL[0
lSiSPX[NNO)nsX-N0
(W,dЏNhV-N lal g5uQogvlSiONNhV-Nn=0
4 . 7 . 1 ޏcYc5ug5ulS5uT vcN
^hQN-Oo` +T g N*NcN ޏcYc5ug09eSR.P / F vMOn cNSޏcYc5ugۏL5ulS&{S" T~ހT&{S 04 m m [hQc4YޏcYc5ug0
1 ޏcc^ kp~~r
2 ޏcc^ 0W~ўr
S_(uYc5ugۏL~ހ5uT[e 1uNc4Yޏc N*N5 0 'kYv5u;N5ugv^T0O(uY(W5ug_{N~hg/f&T&{TV[[hQĉ[NS5ux|Q['`^(uĉ[ nxO[hQO(u_{[@b gޏcvY #
4 . 8 ~ހTS b
4 . 8 . 1 OS~ހT\ NvKm
:NNOS~ހTSpe g_ PRyrkv_Tog(W>f_\ N[vKm~ހcRTT0_TogǏN(uvޏc~\я1 s| N_TcSvޏ0
la{b{>e_Tog\_R}Y~ހ`m
N(ug4YSbyR5ug 2bk_cOW~ހcRTǏ z-NMQN5ugvccS g\_TogޏcT ~ހcRTMbۏL
_Tog'}'}ޏcM u l t i p o r a t o r 0
\_TogR g'Y~5 0 u l v~ހ`m >e(W>f_\ N0la(W5ughTV\_nR~ހ`mXR5ugv~ހpe0
9YGrSN(W>f_\s^S
NV[_Tog _NS(uNV[sGr0
\_TogTN(uޏc~ޏcweg0
Z&q5ugT~ހ0
cRS T A R T c cHQ[vSpeۏL~ހcRT0SN(W\ NRNvKm0
_Togv g'YleQSOy'Y~:N2 0 0 u l 0
]~nx[vSpeSvc(uNeTog0
4 . 8 . 2 nm_Tog
(umt-NvS4lQm_TogQ }VkYuv~ހkYOirS(ugo7RP[N~db5ug0nmǏ z-N_{Oc5ugݍ
NS0 SN(u7 0 R|nmNR r^qǏ z0
4 . 8 . 3 eTog
'Yϑ~ހvTSǑ(ueTog0
eTogv~g
eTog1u N*NogP[T N*N1uv^Re Ns~v-N_N$NR~b0 NsS:N5ug0ev NNSeb__s~-N_N0$NRvNb'}0ogP[T8h_vzzS[~2 5 0 u l v7hT0
eTogǏqQtvc4YNTcSvޏ0
e N5ugݍ'Y~:N2 0 0 m 0
V5 \ NeTog5ug
la\_YteTog~gRb_cOW5ugOg'Yq_Td\O z^ ُy`Q N eTog
NO(u
4 . 8 . 4 eTogv
N7hSneQ
eTogS[~2 5 0 u l v7hT _{OmSOhQ萠RogP[v^0TX
N
N^non &TRO bq_T7hTReQeTog-N0ll_NOMNO[vHes0
8h_ceQST
N>envogP[ 6qT\_b'}eQMO0b'}Ǐ z-N -NfYN^萄vv~ހ`m
NGS0
b'}8h_e nxOl gllb_b0
v0RT[bMR OeTogzv>en(WqQtޏchV
N
4 . 8 . 5 TS~ހ`mcS
\TceQi3z[0WceQNhV-N0
\[\}YveTogޏc0RTqQtcS
N el9 0 ^T'}0
cHQ[vSpeۏLT5 . 3 R 0
\_el9 0 ^~gRTS NeTog0
WveT\_Sb _T[0
\_b _8h_0
(u\ϑS_vW{QWm N8h_
N|Dv~ހ`m0
og-Nv~ހ`mTm Nv`m6eƖT c \Oۏ NekYt0
4 . 8 . 6 eTogvnmTmk
2bk~ހTQmr^T|D (W[T(uSyP[4lnmogP[T8h_0
Ygalg%N͑ _{(uXSv(uE d i s o n i t e S u p e r mQeTog b(u^8^gov7RP[nm0(u7RP[nm O7RmeTN N5ug ~eT N &TRMOv N5ugO[eTog
NO(u0
(u7 0 R|nmmk0nmTzv>e(WẽeppvsX-Nr^q r^qTeTogS͑
YO(u0
4 . 9 SbpSޏcSbpS:gS b
M u l t i p o r a t o r SQk*N[SbpSbJT0SbpSbJT
NN+T g[vSpeNSՋve gTe ؏SRQQm0Sm^0~ހ*hI{vQNvpencOo`0Qe QOo`_NOSbpSQeg0
N TSbpS^(uOP[R>NY N
2NTSbpS:gSNcNNhVTevSbpS:gcS0_NSǏP C @b gv[penc ُ N*Ne6RhVv~9 - i n ci 'S0 0 1 3 6 1 0 . 5 2 5
E p p e n d o r f A G cON NpOeSbpS:g0:N|Q[I B M SbpS:gvD I P lbcňn_NR>NY N
5 d\O!j_
5 . 1 w8h~ހ!j_
ُy!j_v&{S:N"0
,{ Nek [Spe
9hnc cM O D E .leQ w8h~ހr`0
cS E T . bSpe bvSpeO g NR~Qs 0
(u
N N{4Y. cBl9eRSpev'Y\0
9hnc cS E T .T{4YO9evQNSpe0
\PbkSpe[ S_e[vSpe gHe
N_PX[0
w8h~ހ5ulS!j_O\>f>NO0
vsQSpeN'YeSOb__Qs0
U 5uS USMOV 0SN1 V XE^(W2 0 - 1 0 0 V VQXQbN1 0 v vXE^(W2 0 1 0 0 V VQXQ N1 0 0 V XE^(W1 , 0 0 0 1 , 2 0 0 V VQXQ0
e USMOu s _y 0SN5 u s XE^(W1 5 5 0 0 u s VQXQ0
n [-N Q!kpe0SNN1 9 9 !k0k$N!k Q-Ne:N1 R0
la][vSpesSS^(u09hnc[vSpe NhVQL{ Qf~ VdkSN cgqeQvSpeL~c>e5uf~cpepQf~ 0T!j_^(uv/fel Q5 . 4 R 0
,{Nek _Y5ulS
cS T A R T . _Y5ulS0
C h a r g e EQ5u Qs(WO\U^,{ NL0
EQ5uǏ z~_gT _Y>e5uǏ z O\U^,{ NLO g N*NꕨRRNc:y0
傾[NYN N*Nv Q W a i t TiRYOe\Qs(WO\U^
N0
[~_gT NhVOSQ$NXc:y O\U^V
Y0RHQ[T]O(uvLub0
N[~_g _Yv^eONRyvb__Qs(W g^L Ǐ9 9 RbcRNUO c ُ*NOo`ONO\U^
Nm1Y0
5 . 2 ~̃Tuk!j_S b
ُy!j_v&{S:N%0
,{ Nek [/ O9eSpe
9hnc cM O D E .leQ~̃Tukd\Or`0
cS E T . b5uSU Spe bvSpeO g NR~Qs 0
(u
N N{4Y. cBl9eRSpev'Y\0
\PbkSpe[ S_e[vSpe gHe
N_PX[0
~̃Tuk5ulS!j_O\>f>NO0
vsQSpeN'YeSOb__Qs0
U 5uS USMOV 0SN1 0 V XE^(W2 0 0 - 1 0 0 0 V VQXQbN1 0 0 v vXE^(W1 0 0 0 2 5 0 0 V VQXQ0
^~e USMOm s ky 0b5 . 0 m s 0
,{Nek _Y5ulS
cS T A R T . _Y5ulS0
C h a r g e ( EQ5u Qs(WO\U^,{ NL0
EQ5uǏ z~_gT _Y>e5uǏ z O\U^,{ NLO g N*NꕨRRNc:y v^SQ$NXTX0
[~_gT NhVOSQ$NXc:y [vSpeQs(WO\U^
N [E^(uvSpeU a Ta Qs(WO\U^,{ NL0
U a [EKmϑvO(u5uS USMOOyrV
a [EO(u Qe USMOm s ky ed"0 . 8 m s :N0 . 8 m s 0
N[~_g _Yv^eONRyvb__Qs(W g^L Ǐ9 9 RbcRNUO c ُ*NOo`ONO\U^
Nm1Y0
5 . 3 ~ހT!j_S b
5 . 3 . 1 R~ހcR
~ހcRRR+R(WO\U^,{NLT,{VLN&{SU ( ~ QMR TU ( ( ~ QT h:y0
,{ Nek [Spe
9hnc cM O D E .leQ~ހTr`0
cS E T . bSpe bvSpeO g NR~Qs 0
(u
N N{4Y. cHQBl9eRSpe'Y\0U ( ~ U ( ( ~ U Spe:N0 Ry
NgbL0ُ7hO_U ( ~ U ( ( ~ SUSrbO!ko;m
N o;mT Q0
\PbkSpe[ S_e[vSpe gHe
N_PX[0
No;m QU ( TU ( ( ~ o;m NU ( ~ ۏL~ހcRO\>f>NO0
vsQSpeN'YeSOb__Qs0
U ( ~ U ( ( ~ NAm5uS USMOV 0SN:N0 V bN0 . 1 V XE^(W1 . 0 - 1 0 V VQXQ0
t cRc~e USMOs y 0SN5 s XE^(W0 9 5 s VQXQ0
U 5uSel Q USMOv 0SN:N0 bN1 V XE^(W5 - 1 0 0 V VQXQ0 bN1 0 V XE^(W1 0 0 - 3 0 0 V VQXQ0
t Qc~e USMOu s _y 0SN5 u s XE^(W0 - 3 0 u s XQ0
n Q!kpe0SN!kX9 9 !k0
,{Nek _Y~ހcR
cS T A R T .S~ހcR5uSU ( ~ 0
Qs(WO\U^,{VL NAm5uS\O(u0
[~_gT NhVOSQ$NXc:y O\U^V
Y0RHQ[T]O(uvLub0N[~_g _Yv^eONRyvb__Qs(W g^L Ǐ9 9 RbcNUO c ُ*NOo`ONO\U^
Nm1Y0
5 . 3 . 2 ~ހTek
,{ Nek [/ 9eSSpe
cS E T . bSpe bvSpeO g NR~Qs 0
(u
N N{4Y. cHQBl9eRSpe'Y\09hnc bvSpeU ( ~ TU ( ( ~ TSN(We~ހcRv`Q NۏL _NSN N*Nb$N*N bvcRR~TۏL0
\PbkSpe[ S_e[vSpe gHe
N_PX[0
wQ gU ( ~ U ( ( ~ o;m~ހcRv5uT!j_O\>f>NO0
ecR5uS QU ( ~ ~ހT!j_O\>f>NO0
vsQSpeN'YeSOb__Qs0
U 5uS USMOV 0SN:N0 _NSN1 0 V XE^(W5 1 0 0 V VQXQ bN1 0 V XE^(W1 0 0 3 0 0 V VQXQ0
t Qc~e USMOu s _y 0SN5 u s XE^(W0 3 0 0 u s VQXQ0
U ( ~ U ( ( ~ NAm5uS USMOV 0SN:N0 bN0 . 1 V XE^(W1 . 0 1 0 V VQXQ0
n Q!kpe0SN!kX9 9 !k0
,{Nek _Y~ހT
cS T A R T .S~ހT0
C h a r g e Qs(WO\U^,{VL c:yck(WۏLd\O0
gbLd\O gNAm5uSU ( ~ \O(u NAm5uSN&{S^h:yck&_l 0
~ހcRe0
9 5 y TsS _Y>e5u ُeO(WO\U^,{VL g*NꕨR0傾[v!kpen 'YN1 RǏ1 yTOQ!kgbL>e5u0
>e5u~_gT NAm5uSU ( ( ^\O(u0
9 9 y te*N[@biRe>f:y(WO\U^ g^L0
[~_gT NhVOSQ$NXc:y O\U^V
Y0RHQ[T]O(uv܃USLub0
N[~_g _Yv^eONRyvb__Qs(W g^L Ǐ9 RbcNUO c ُ*NOo`ONO\U^
Nm1Y0
laS_U ( ~ U ( ( ~ :N0 e8^`Q N(uN~ހTvel Q_NS(uN5ulS0ُe5ulSNT!j_TvQvsQSpeۏL0(W^T!j_ N el Q
N^(u0
6 c:y
Qe M u l t i p o r a t o r NhVOSQ#Xc:y O\U^
N_NOQsc:ybǏSbpS:gSbpSQeg0ُNOo`SbNhVvT^(uv01
4 9 SS uN~̃!j_[ 1
7 SS uN w8h~ހ!j_ 1
8 S uN~ހT[0
x Oo` SV ㉳Qel 0 1 N o D i s p l a y >f:yhVOWN NO^FUT|0 0 2 N o R T C / N V R A M NhVQev5uP[ g NO^FUT|0
0 3
H i g h C u r r e n t
(W>e5uǏ z5uAm*Yؚ MNOO(unmv O['`
hg (uv5uQog/f&Tcknx0
0 4
N o C u v e t t e
*gceQ5uQog ceQ5uQog
͑e/TRNhV
\5uQogic0R^0 0 5 T i m e o u t C h a r g e (WRMveQ5u[RShV
N]\O NO^FUT|0 0 6 T i m e o u t C h a r g e (WS
Y QǏ z-N 5u[RShV(W$N*N Qel]\O MNOO(unmv O['`
hg (uv5uQog/f&Tcknx0
0 7
S h o r t 5u[RShV[hQzzN
Nnx[/f&TgbL0CQNv gP'`(WN5u[RShV
N/f QhV0 MNOO(unmv O['`
hg (uv5uQog/f&Tcknx0 0 8 L o w R e s i s t a n c e 5uQogv5uAm*Y'Y el~cNAm5uS0 MNOO(unmv O['`0
0 9 T i m e o u t M e a s u r i n g ~̃!j_-NKmϑ~bk NO^FUT|0 ~̃!j_ a = 0 . 8 m s , U a i s c o n s i d e r a b l y l o w e r t h a n t h e p a r a m e t e r U w h i c h h a s b e e n s e t . 5uQog-N gkp
(W>e5uǏ z5uAm*Yؚ
b/g O(u5ugݍ'Yv5uQog0
MNOO(unmv O['`0
NO^FUT|0
7 NhVv~b
NhV
N ~8^~b NhV~ FU #OOT.UT
gR0
7 . 1 mk
mkMRHQRe5un0M u l t i p o r a t o r NhV@b gR SbNTޏc~_{dbmk05uQog^_NSۏLUmmk0FO/f
N^\te*NNhVۏLUmk N2bkmkBRۏeQNhVQ0
7 . 2 nm
nmM u l t i p o r a t o r NhVMRRe5un0Ol gmSOۏeQNhV 2bk b5uwTNhVP0(ugTnmBRdbUoTݔ6RMO 6qT(ur^^dr^0
laybkO(uNUOwQ gP'`0 g:gvbidd'`vSalBRb
NIQBR0
8 b/gpenc
5uS/ s: 1 0 0 - 2 4 0 V ( 1 0 % , 5 0 - 6 0 H z
Oi: T 0 , 1 . 0 A - 5 * 2 0 m m ( 2 p c s )
Rs: 6 0 W
ǏS{|W: I I
Ob~+R: I
alg z^: 2
NR!j_5uSV:
w8h!j_: 2 0 - 1 0 0 V , XE^ 1 V
1 0 0 - 1 , 0 0 0 V , XE^1 0 V
1 , 0 0 0 - 1 , 2 0 0 V , XE^1 0 0 V
$N!k Q 1 R
~ހT: 0 , 5 - 1 0 0 V , XE^ 1 V
1 0 0 - 3 0 0 V , XE^ 1 0 V
$N!k Q: 1 y
NAm5uS: 0 , 1 . 0 - 1 0 V , XE^ 0 . 1 V
~̃: 2 0 0 - 1 , 0 0 0 V , XE^ 1 0 V
1 , 0 0 0 - 2 , 5 0 0 V , XE^ 1 0 0 V
eY!kQ
5u[: 1 0 u F , 2 , 5 0 0 V Q>e5u
5u;: 6 0 0 ( v^T
5 6 ( s 2NT
EQ5ue: ( 3 0 y
Q!kpe ( n ) : 0 - 9 9
>e5ue:
w8h~ހ: 1 5 - 5 0 0 u s , XE^ 5 u s , cpepQl
~̃: ؞ 5 m s , &^ N 3 . 3 k (
KmՋT>f:yve 0 . 8 - 6 . 0 m s
cRe: 0 - 9 5 s , XE^ 5 s
Qe:
~ހT: 0 - 3 0 0 u s , XE^ 5 u s , el
R S 2 3 2 cS
sX)n^: gؚ 4 0 !
v[n^: g'Y. 8 0 %
͑ϑ: gY 5 . 5 k g ( ONhV!j_ [
'Y\: [: 2 5 . 2 c m
m: 3 4 . 2 c m
ؚ: 1 2 . 0 c m
NhV~ v^1uU L TC S A cCgc U L : E 1 5 8 0 8 9 0
9 -Oo`
vU_S 4 3 0 8 0 0 0 . 0 1 5 M u l t i p o r a t o r b a s i c v e r s i o n , f o r e u k a r y o t i c c e l l s , 1 0 0 - 2 4 0 V , 5 0 - 6 0 H z 4 3 0 8 0 0 0 . 0 2 3 M u l t i p o r a t o r , e u k a r y o t i c c e l l s , a n d m o d i f i c a t i o n l e v e l b a c t e r i a m o d e 4 3 0 8 0 0 0 . 0 3 1 M u l t i p o r a t o r , e u k a r y o t i c c e l l s a n d m o d i f i c a t i o n l e v e l c e l l f u s i o n 4 3 0 8 0 0 0 . 0 4 0 M u l t i p o r a t o r , e u k a r y o t i c c e l l s a n d m o d i f i c a t i o n l e v e l b a c t e r i a m o d e a n d c e l l f u s i o n DN Qm 4 3 08 070.501Hypo-osmolar buffer for electroporation (PH), 100 ml4308 070.510Iso-osmolar buffer for electroporation (PI), 100 ml4308 070.528Hypo-osmolar buffer for cell fusion (FH), 100 ml4308 070.536Iso-osmolar buffer for cell fusion (FI), 100 ml 5uQog 4 3 0 7 0 0 0 . 5 6 9 1 m m g a p w i d t h , a l u m i n u m , s t e r i l e , 5 0 p c s . 4 3 0 7 0 0 0 . 5 9 3 2 m m g a p w i d t h , a l u m i n u m , s t e r i l e , 5 0 p c s . 4 3 0 7 0 0 0 . 6 2 3 4 m m g a p w i d t h , a l u m i n u m , s t e r i l e , 5 0 p c s . 4 3 0 8 0 7 8 . 0 0 6 C u v e t t e s t a n d f o r 1 6 e l e c t r o p o r a t i o n c u v e t t e s 4 3 0 8 0 7 0 . 0 7 2 I n s e rt for electroporation cuvettes4308 021.004Insert (electroporation / electrofusion) for connecting to external electrodes4308 012.005Insert for helix fusion chambers4308 014.008Helix fusion chamber (gap between electrodes: 0.2 mm)4308 030.003Micro-fusion chamber (gap between electrodes: 0.2 mm)4308 024.003Insert for micro-fusion chambers4308 017.007Stand for 10 helix fusion chambers0013 608.148Thermal printer DPU-4140013 608.172Power unit (230 V) for thermal printer DPU-4140013 608.164Power unit (115 V) for thermal printer DPU-4146547 001.018Thermal paper (10 rolls)0013 610.517Connecting cable (9-pin socket, 9-pin plug, cable guide 1:1)0013 610.525Zero-modem cable for PC connection (9-pin socket, 9-pin socket)0013 610.533Zero-modem cable for matrix printer (9-pin socket / 25-pin plug)4308 010.002Conversion kit for mode for bacteria (to be installed by SERVICE)4308 011.009Conversion kit for mode for cell fusion (to be installed by SERVICE)
PAGE
PAGE 23
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